Calcium/ganglioside-dependent protein kinase activity in rat brain membrane.
作者： James R. Goldenring , Laura C. Otis , Robert K. Yu , Robert J. DeLorenzo
DOI: 10.1111/J.1471-4159.1985.TB08748.X
关键词:
摘要: The effects of gangliosides on phosphorylation were studied in rat brain membrane. Gangliosides stimulated only the presence Ca2+ with major phosphoproteins 45,000, 50,000, 60,000, and 80,000 daltons high-molecular-weight species. In addition, inhibited three proteins molecular weights 15,000, 20,000, 78,000 daltons. two low-molecular-weight comigrated myelin basic proteins. Ganglioside stimulation was dependent formation a Ca2+-ganglioside complex since calcium salt maximally. Disialo trisialo more potent stimulators kinase activity than monosialo GM1 X GD1a most activator tested. Asialo-GM1, cerebroside, sialic acid, neuraminyllactose, sulfatide, acidic phospholipids phosphatidylserine phosphatidylinositol did not stimulate activity. Ca2+-dependent, ganglioside-stimulated qualitatively similar to pattern for calmodulin-dependent phosphorylation. However, while an IC50 10 microM trifluoperazine, 200 trifluoperazine. These results indicate that have membrane-associated activities suggest complexes are membrane
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sci-hub.se 参考文章(38)
Bruno Maggio, Federico A. Cumar, Ranwel Caputto, Configuration and interactions of the polar head group in gangliosides Biochemical Journal. ,vol. 189, pp. 435- 440 ,(1980) , 10.1042/BJ1890435
S Ando, R K Yu, Isolation and characterization of a novel trisialoganglioside, GT1a, from human brain. Journal of Biological Chemistry. ,vol. 252, pp. 6247- 6250 ,(1977) , 10.1016/S0021-9258(17)39945-3
Y Takai, A Kishimoto, Y Iwasa, Y Kawahara, T Mori, Y Nishizuka, Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. Journal of Biological Chemistry. ,vol. 254, pp. 3692- 3695 ,(1979) , 10.1016/S0021-9258(18)50638-4
Ying Ming Lin, Yung Pin Liu, Wai Yiu Cheung, Cyclic 3' : 5'-Nucleotide Phosphodiesterase PURIFICATION, CHARACTERIZATION, AND ACTIVE FORM OF THE PROTEIN ACTIVATOR FROM BOVINE BRAIN Journal of Biological Chemistry. ,vol. 249, pp. 4943- 4954 ,(1974) , 10.1016/S0021-9258(19)42412-5
E G Bremer, S Hakomori, D F Bowen-Pope, E Raines, R Ross, Ganglioside-mediated modulation of cell growth, growth factor binding, and receptor phosphorylation. Journal of Biological Chemistry. ,vol. 259, pp. 6818- 6825 ,(1984) , 10.1016/S0021-9258(17)39801-0
Lars Svennerholm, Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochimica et Biophysica Acta. ,vol. 24, pp. 604- 611 ,(1957) , 10.1016/0006-3002(57)90254-8
V. Niggli, E.S. Adunyah, E. Carafoli, Acidic phospholipids, unsaturated fatty acids, and limited proteolysis mimic the effect of calmodulin on the purified erythrocyte Ca2+ - ATPase. Journal of Biological Chemistry. ,vol. 256, pp. 8588- 8592 ,(1981) , 10.1016/S0021-9258(19)68885-X
B C Wise, R L Raynor, J F Kuo, Phospholipid-sensitive Ca2+-dependent protein kinase from heart. I. Purification and general properties. Journal of Biological Chemistry. ,vol. 257, pp. 8481- 8488 ,(1982) , 10.1016/S0021-9258(18)34357-6
R.W. Wrenn, N. Katoh, B.C. Wise, J.F. Kuo, Stimulation by phosphatidylserine and calmodulin of calcium-dependent phosphorylation of endogenous proteins from cerebral cortex. Journal of Biological Chemistry. ,vol. 255, pp. 12042- 12046 ,(1980) , 10.1016/S0021-9258(19)70241-5
Robert W. Ledeen, Robert K. Yu, Gangliosides: structure, isolation, and analysis. Methods in Enzymology. ,vol. 83, pp. 139- 191 ,(1982) , 10.1016/0076-6879(82)83012-7