Antisense DNA oligonucleotides II: The use of matrix‐assisted laser desorption/ionization mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotides
作者： Thomas Keough , Timothy R. Baker , Roy L. M. Dobson , Martin P. Llacey , Timothy A. Riley
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摘要: Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has been used to measure accurately the molecular masses of synthetic methylphosphonate oligodeoxyribonucleotides, up 18 nucleotides in length. A simple method developed for complete sequence verification these compounds, which are intractable by classical means. Sequencing from 5' end molecule is possible because inefficiencies procedure. Complementary information 3' can be obtained partial hydrolysis backbone.
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Franz Hillenkamp, Michael Karas, [12] Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization Mass Spectrometry. ,vol. 193, pp. 280- 295 ,(1990) , 10.1016/0076-6879(90)93420-P
R. Nelson, M. Rainbow, D. Lohr, P Williams, Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions Science. ,vol. 246, pp. 1585- 1587 ,(1989) , 10.1126/SCIENCE.2595370
Ronald C. Beavis, Brian T. Chait, K. G. Standing, Factors affecting the ultraviolet laser desorption of proteins Rapid Communications in Mass Spectrometry. ,vol. 3, pp. 233- 237 ,(1989) , 10.1002/RCM.1290030708
K. Strupat, M. Karas, F. Hillenkamp, 2,5-Dihydroxybenzoic acid: a new matrix for laser desorption—ionization mass spectrometry International Journal of Mass Spectrometry and Ion Processes. ,vol. 111, pp. 89- 102 ,(1991) , 10.1016/0168-1176(91)85050-V
R. C. Beavis, B. T. Chait, Rapid, sensitive analysis of protein mixtures by mass spectrometry Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 6873- 6877 ,(1990) , 10.1073/PNAS.87.17.6873
Michael. Karas, Franz. Hillenkamp, Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons Analytical Chemistry. ,vol. 60, pp. 2299- 2301 ,(1988) , 10.1021/AC00171A028
M. Karas, U. Bahr, A. Ingendoh, E. Nordhoff, B. Stahl, K. Strupat, F. Hillenkamp, Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometry Analytica Chimica Acta. ,vol. 241, pp. 175- 185 ,(1990) , 10.1016/S0003-2670(00)83645-4
Ronald C. Beavis, Brian T. Chait, K. G. Standing, Matrix‐assisted laser‐desorption mass spectrometry using 355 nm radiation Rapid Communications in Mass Spectrometry. ,vol. 3, pp. 436- 439 ,(1989) , 10.1002/RCM.1290031208
T. Huth-Fehre, J. N. Gosine, K. J. Wu, C. H. Becker, Matrix-assisted laser desorption mass spectrometry of oligodeoxythmidylic acids Rapid Communications in Mass Spectrometry. ,vol. 6, pp. 209- 213 ,(1992) , 10.1002/RCM.1290060312
Louis J. Romano, Robert J. Levis, Nondestructive laser vaporization of high molecular weight, single-stranded DNA Journal of the American Chemical Society. ,vol. 113, pp. 9665- 9667 ,(1991) , 10.1021/JA00025A038