Interleukin-1beta induces bradykinin B2 receptor gene expression through a prostanoid cyclic AMP-dependent pathway in human bronchial smooth muscle cells.
作者： Yves Landry , Jean-Pierre Gies , Didier Scherrer , Fabien Schmidlin , Laurent Daeffler
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摘要: We investigated the hypothesis that inflammatory mediators such as interleukin-1beta (IL-1beta) might be responsible for hyperreactivity of asthmatic patients to bradykinin. In cultured human bronchial smooth muscle cells, IL-1beta elicited a rapid and transient increase in density bradykinin B2 receptors without affecting their affinity ligands. The was correlated an enhancement inositol phosphate formation by bradykinin, indicating its relevance contractile response cells receptor related mRNA level corresponding 5-fold transcriptional rate lengthened half-life mRNA. These effects were largely inhibited indomethacin, suggesting involvement prostanoid pathway transduction process. An prostaglandin E2 levels preceded increase, confirming this involvement. Moreover, led cAMP formation. propose predominant stimulate transcription gene major mechanism involved hyperresponsiveness
参考文章(39)
Terasaki Wl, Brooker G, Harper Jf, Moylan Rd, Radioimmunoassay of cyclic AMP and cyclic GMP. Advances in cyclic nucleotide research. ,vol. 10, pp. 1- 33 ,(1979)
Alain Israë, Signal transduction: IκB kinase all zipped up Nature. ,vol. 388, pp. 519- 521 ,(1997) , 10.1038/41433
C D Strader, R W Ransom, T MacNeil, A W Derrick, J G Menke, J A Borkowski, K K Bierilo, J F Hess, D L Linemeyer, K A Schneck, Expression cloning of a human B1 bradykinin receptor. Journal of Biological Chemistry. ,vol. 269, pp. 21583- 21586 ,(1994) , 10.1016/S0021-9258(17)31844-6
L Borish, R J Martin, J Dishuck, W R Beam, J J Mascali, L J Rosenwasser, Detection of alveolar macrophage-derived IL-1 beta in asthma. Inhibition with corticosteroids. Journal of Immunology. ,vol. 149, pp. 3078- 3082 ,(1992)
L L Brunton, R A Wiklund, P M Van Arsdale, A G Gilman, Binding of (3H)prostaglandin E1 to putative receptors linked to adenylate cyclase of cultured cell clones. Journal of Biological Chemistry. ,vol. 251, pp. 3037- 3044 ,(1976) , 10.1016/S0021-9258(17)33496-8
N Nishigaki, A Ichikawa, M Negishi, Two Gs-coupled prostaglandin E receptor subtypes, EP2 and EP4, differ in desensitization and sensitivity to the metabolic inactivation of the agonist Molecular Pharmacology. ,vol. 50, pp. 1031- 1037 ,(1996)
M J Berridge, R M C Dawson, C P Downes, J P Heslop, R F Irvine, Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides. Biochemical Journal. ,vol. 212, pp. 473- 482 ,(1983) , 10.1042/BJ2120473
J. C. W. Mak, El-Bdaoui Haddad, J. Rousell, P. J. Barnes, Transforming growth factor-beta 1 induces transcriptional down-regulation of m2 muscarinic receptor gene expression. Molecular Pharmacology. ,vol. 49, pp. 781- 787 ,(1996)
Timothy A. Bird, Heather D. Schule, Pamela B. Delaney, John E. Sims, Bettina Thoma, Steven K. Dower, Evidence that map (mitogen-activated protein) kinase activation may be a necessary but not sufficient signal for a restrictive subset of responses in IL-1-treated epidermoid cells Cytokine. ,vol. 4, pp. 429- 440 ,(1992) , 10.1016/1043-4666(92)90003-A
B.G. Simonsson, B.-E. Skoogh, N.P. Bergh, R. Andersson, N. Svedmyr, In vivo and in vitro effect of bradykinin on bronchial motor tone in normal subjects and patients with airways obstruction. Respiration. ,vol. 30, pp. 378- 388 ,(1973) , 10.1159/000193051