Detection of alveolar macrophage-derived IL-1 beta in asthma. Inhibition with corticosteroids.

摘要: This study investigated alveolar macrophage function in asthma as assessed by the presence and source of cytokine IL-1 beta. Bronchoalveolar lavage (BAL) fluid cell pellets were obtained at 4 a.m. from a cohort asthmatic subjects. Normal subjects used controls. Asthmatic BAL contained 57.0 +/- 5.9 pg/ml beta determined ELISA. No could be identified control group. represented synthesis cells airway beta-specific mRNA transcripts pellets. Inasmuch are known to present for only short time period after activation (approximately 0.5 16 h), represents recent cellular activation. Using technique situ hybridization, found located exclusively within macrophages, mean 40 14% activated lavage. Corticosteroids efficacious treatment asthma. Administration single 50-mg dose prednisone 3 p.m. resulted diminished protein concentration (28.3 5.7 pg/ml; p < 0.01 compared baseline) completely abrogated expression. In summary, these studies demonstrate that is synthesized airways suggest may mediator Inhibition an additional mechanism efficacy corticosteroids