Inhibition of eosinophilic inflammation in allergen-challenged, IL-1 receptor type 1-deficient mice is associated with reduced eosinophil rolling and adhesion on vascular endothelium.

摘要: To determine the relative in vivo importance of IL-1 release after allergen challenge to subsequent endothelial adhesion and recruitment eosinophils, authors used ovalbumin sensitization inhalation induce airway eosinophilia receptor type 1-deficient control wild-type mice. Bronchoalveolar lavage (BAL) eosinophil mice challenged with (24.3% ± 6.3% BAL eosinophils) was significantly reduced compared (63.7% ± 2.5% eosinophils). whether inhibition vascular endothelium contributed mice, intravital microscopy visualize rolling firm fluorescence-labeled mouse eosinophils microvasculature allergen-challenged mesentery. Eosinophil rolling, endothelium, transmigration across (peritoneal were inhibited Overall, these studies demonstrate that cytokines such as IL-1, released challenge, are important induction cell adhesiveness, a prerequisite for circulating eosinophils. (Blood. 2000;95:263-269)