Morphological recovery of axotomized goldfish retinal ganglion cells in an environment known to prevent retinotopic refinement of their regenerated tectal arbors

摘要: Axonal injury provokes well-characterized morphological changes in goldfish retinal ganglion cells. These reach a peak as the regenerating axons restore grossly retinotopic projection map to tectum, and then regress is refined by mechanism involving locally-correlated activity. The aim of this study was look for any interdependence between recovery refinement. Stroboscopic light used keep regenerated optic arbors non-retinotopic locations 70 days after nerve cut lens ablation. Controls were kept constant or diurnal light, both which allow refinement retinotectal map. Nucleolar frequency, perikaryal area nuclear indices neuronal recovery, cell counts performed. After 35 nucleoli axotomized cells had increased size, prominence number, nucleus cytoplasm roughly doubled area. days, these features almost returned normal not only but stroboscopic well. A small significant loss, averaging 13.4-14.7%, seen ablation regardless stage regeneration (35 days) lighting. Evidently, independent refinement, known be no further advanced than light.