Characterization of the transposon carrying the STII gene of enterotoxigenic Escherichia coli
作者： S. T. Hu , C. H. Lee
DOI: 10.1007/BF00330485
关键词:
摘要: The Escherichia coli enterotoxin STII gene is carried by Tn4521. terminal repeats of Tn4521 are composed IS2 sequences; however, neither repeat a complete IS2. In order to determine how this seemingly defective transposon could transpose, mutations were generated within the regions essential for transposition. left region was found be non-essential, but right area demonstrated crucial Within an open reading frame (ORF), capable encoding 159 amino acid protein, which shown frameshift mutation analysis required This protein may transposase A pair 11 bp sequences flanking ORF also important. part sequence, and located about 300 upstream from sequence region. results study suggest that functional including plus intervening transposable element responsible
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