Collagen metabolism and basement membrane formation in cultures of mouse mammary epithelial cells: Induction of ‘Assembly’ on fibrillar type I collagen substrata☆

摘要: Abstract Collagen metabolism was compared in cultures of mouse mammary epithelial cells maintained on plastic or fibrillar type I collagen gel substrata. The accumulation dialysable and non-dialysable [ 3 H]hydroxyproline the identification collagens produced suggest no difference between substrata allover rates synthesis degradation. proportion H]collagen which accumulates monolayers collagen, however, markedly exceeds that plastic. Cultures deposit a sheet-like layer extracellular matrix materials surface fibres. Immunoprecipitation labelled extracts, electrophoresis, indirect immunofluorescence immunoperoxidase techniques reveal presence IV along with laminin heparan sulfate proteoglycan this layer, excess over amounts detectable cultured Transformed produce accumulate more H]collagen, yet are less effective basement membrane formation than normal cells, indicating alone effect interstitial thereupon do not suffice. Thus, exogenous appears to enhance, but is sufficient for proper assembly collagenous components near basal cell surface.