Mutations of the Ki-ras oncogene in carcinoma of the endometrium.

摘要: Mutations of the Ki-ras oncogene in endometrial carcinoma have been reported Japan, but prevalence and clinical significance such mutations United States remains unclear. DNA extracted from paraffin sections 112 carcinomas endometrium was amplified by polymerase chain reaction with mismatched primers that generated a BstNI recognition site wild-type codon 12. Loss this indicating 12 observed 13 tumors (11.6%), including 11 endometrioid carcinomas, one undifferentiated carcinoma, carcinosarcoma. None 17 papillary serous-clear cell contained mutations. These were confirmed characterized direct sequencing. We found no evidence correlation presence stage, grade, depth invasion, or outcome. Our results indicate may be less prevalent than Japan.