VEGF and Angiopoietins Promote Inflammatory Cell Recruitment and Mature Blood Vessel Formation in Murine Sponge/Matrigel Model

摘要: A key feature in the induction of pathological angiogenesis is that inflammation precedes and accompanies formation neovessels as evidenced by increased vascular permeability recruitment inflammatory cells. Previously, we other groups have shown selected growth factors, namely endothelial factor (VEGF) angiopoietins (Ang1 Ang2) do not only promote angiogenesis, but can also induce response. Herein, given a pro-inflammatory environment, addressed individual capacity VEGF to mature identify different types cells accompanying angiogenic process over time. Sterilized polyvinyl alcohol (PVA) sponges soaked factor-depleted Matrigel mixed with PBS, VEGF, Ang1, or Ang2 (200 ng/200 µl) were subcutaneously inserted into anesthetized mice. Sponges removed at day 4, 7, 14, 21 post-procedure for histological, immunohistological (IHC), flow cytometry analyses. As compared PBS-treated sponges, three factors promoted cells, mainly neutrophils macrophages, lesser extent, T- B-cells. In addition, they more potent rapid (ECs) maturation (ensheating smooth muscle around ECs) neovessels. Thus, autocrine/paracrine interaction among combination provides suitable microenvironment blood vessels. J. Cell. Biochem. 116: 45–57, 2015. © 2014 Wiley Periodicals, Inc.