Comparative proteomic analysis of Saccharomyces cerevisiae under different nitrogen sources.

摘要: Abstract In cultures containing multiple sources of nitrogen, Saccharomyces cerevisiae exhibits a sequential use nitrogen through mechanism known as catabolite repression (NCR). To identify proteins differentially expressed due to NCR, proteomic analysis S. S288C under different source conditions was performed using two-dimensional gel electrophoresis (2-DE), revealing 169 candidate protein spots. Among these spots, 121 were identified by matrix assisted laser desorption ionization-time flight/time flight mass spectrometry (MALDI-TOF/TOF). The closely associated with four main biological processes Gene Ontology (GO) categorical analysis. identification the potential and cellular related NCR offer global overview changes elicited sources, providing clues into how yeast adapt nutritional conditions. Moreover, comparing our data corresponding mRNA data, regulated at transcriptional post-transcriptional level could be distinguished. Biological significance cerevisiae, provide growth characteristics generate metabolites. (NCR) process plays an important role for in ordinal utilization sources. can result significant shift metabolic networks. Previous works on primarily focused transcriptomic level. results obtained this study provided atlas proteome triggered would facilitate understanding mechanisms