Repetitive extraction of botryococcene from Botryococcus braunii: A study of the effects of different solvents and operating conditions

摘要: As global temperatures continue to increase the move renewable energy sources is becoming an essential step in achieving a sustainable future. Biofuels from microalgae, while still more expensive produce than fossil fuels, are of great interest for high microalgae biomass growth rates, lipid content, relatively low water requirements, and ability be grown on non-arable land. Botryococcus braunii freshwater green microalga which grows colonies supported by extracellular matrix (ECM) liquid hydrocarbons. Recent studies have investigated efficiency non-destructive situ solvent extraction lipids B. with variety solvents. These encapsulated retaining wall coated sheath amphiphilic fibrils been found act as barrier By carrying out repeated shear environment, we that access ECM can significantly improved. Sustainable botryococcene total productivities respectively 29.9 ± 8.5 71.3 ± 20.3 mg L−1 day−1 were achieved heptane extraction, rate 415 s−1, initial culture density 1.62 ± 0.12 g L−1, contact period 4 h, recovery 3 days. Nutrient supplementation replace those lost emulsion entrained will required maintain process. The combined presence proved effective colony disruption method could pre-treatment followed shear, contact, method.