Emerging Technologies for Genome-Wide Profiling of DNA Breakage.

摘要: Genome instability is associated with myriad human diseases and a well-known feature of both cancer neurodegenerative disease. Until recently, the ability to assess DNA damage-the principal driver genome instability-was limited relatively imprecise methods or restricted studying predefined genomic regions. Recently, new techniques for detecting double strand breaks (DSBs) single (SSBs) next-generation sequencing on genome-wide scale nucleotide resolution have emerged. With these tools, efforts are underway define "breakome" in normal aging Here, we compare relative strengths weaknesses technologies their potential application diseases.