CDKN2A-p53 mediated antitumor effect of Lupeol in head and neck cancer

摘要: The tumor suppressor protein p53 is known to control cell cycle arrest and apoptosis. Lupeol a phytochemical that has been found induce apoptosis in different cancer types through the extrinsic pathway. As yet, however, its role induction of intrinsic pathway head neck not investigated. Here, we aimed at understanding mechanism underlying antitumor effect cancer. on oral laryngeal carcinomas was assessed using two vitro 2D line models (HEp-2, UPCI:SCC-131) and, subsequently, an ex vivo 3D explant culture platform maintains key features native microenvironment. Lupeol-mediated responses delineated MTT, colony formation, flow cytometry, immunofluorescence, Western blotting immunohistochemistry assays. We induced enhanced expression both tested G1 phase. In addition that, following treatment, Bax activated apoptotic (as measured by Caspase-3 cleavage). Interestingly, also trigger up-regulation CDKN2A, but p21, resulting inhibition CyclinD1. impart potent response as defined Ki67 expression, decreased viability concomitant activation (cleavage) Caspase-3. Finally, can re-sensitize primary squamous carcinoma (HNSCC) samples had clinically progressed under Cisplatin treatment regimen. Together, our data indicate may orchestrate bifurcated regulation neoplastic growth cancers serve promising agent for management tumors have platinum-based