Autoantibodies to topoisomerase I (Scl-70): Analysis by gel diffusion, immunoblot, and enzyme-linked immunosorbent assay
作者： Sabine Hildebrandt , Ethan S. Weiner , Jean-Luc Senecal , G.Stephen Noell , William C. Earnshaw
DOI: 10.1016/0090-1229(90)90114-6
关键词:
摘要: Anti-topoisomerase I autoantibodies (anti-topo I, anti-Scl-70) are associated with proximal scleroderma and of prognostic significance in patients Raynaud's disease. To establish a highly sensitive specific system for the detection anti-topo we have investigated sera from 409 controls by Ouchterlony gel diffusion, Western immunoblot on chromosome proteins, solid-phase enzyme-linked immunosorbent assay (ELISA) purified topoisomerase as antigen. The ELISA was more than diffusion technique immunoblot, while may identify additional autoantibodies.
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