Temporal endogenous gene expression profiles in response to polymer‐mediated transfection and profile comparison to lipid‐mediated transfection

摘要: Background Design of efficient nonviral gene delivery systems is limited by the rudimentary understanding specific molecules that facilitate transfection. Methods Polyplexes using 25-kDa polyethylenimine (PEI) and plasmid-encoding green fluorescent protein (GFP) were delivered to HEK 293T cells. After treating cells with polyplexes, microarrays used identify endogenous genes differentially expressed between treated untreated (2 h exposure) or flow-separated transfected (GFP+) treated, untransfected (GFP–) at 8, 16 24 h after lipoplex treatment. Cell priming studies conducted pharmacologic agents alter levels identified determine effect on transfection levels. Differentially in polyplex-mediated compared those DNA carrier-dependent molecular factors. Results Differentially RGS1, ARHGAP24, PDZD2, SNX24, GSN IGF2BP1 2 h; RAP1A ACTA1 8 h; RAP1A, WDR78 16 h; SCG5, ATF3, IREB2 24 h. Pharmacologic altering for GSN, IGF2BP1, PDZD2 RGS1 able increase decrease transgene production. Comparing polyplexes lipoplexes, no common 2-h time point, whereas, 8-h ATF3 HSPA6 similarly expressed. SCG5 PGAP1 only upregulated polyplex-transfected cells. Conclusions The provide targets improving systems, although polyplex dependencies must be considered. Copyright © 2015 John Wiley & Sons, Ltd.