摘要: The invention relates to a nucleic acid construct comprising at least two different regions, wherein the regions are selected of first region encoding for one miRNA stimulating cellular production biomolecu!e in cell, second and/or miRNA-inhibitor suppressing cell death, and third regulating proliferation. further respective method increasing yield biomolecule produced by cultured vitro steps biomolecule, reducing
lens.org 参考文章(15)
Marc Thibonnier, Cell-specific delivery of mirna modulators for the treatment of obesity and related disorders ,(2013)
Lore Florin, Hitto Kaufmann, Michaela Strotbek, Angelika Hausser, Monilola Olayioye, CELL ENGINEERING USING RNAs ,(2013)
Sumanth Polikepahad, Preethi Gunaratne, Lakeisha Monique Batts, Arash Ole Naghavi, Farrah Kheradmand, David Brian Corry, Mirna expression in allergic disease ,(2010)
Christine Aurnhammer, Maren Haase, Nadine Muether, Martin Hausl, Christina Rauschhuber, Ingrid Huber, Hans Nitschko, Ulrich Busch, Andreas Sing, Anja Ehrhardt, Armin Baiker, Universal real-time PCR for the detection and quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy Methods. ,vol. 23, pp. 18- 28 ,(2012) , 10.1089/HGTB.2011.034
John P. Mayginnes, Sharon E. Reed, Heath G. Berg, Elizabeth M. Staley, David J. Pintel, Gregory E. Tullis, Quantitation of encapsidated recombinant adeno-associated virus DNA in crude cell lysates and tissue culture medium by quantitative, real-time PCR. Journal of Virological Methods. ,vol. 137, pp. 193- 204 ,(2006) , 10.1016/J.JVIROMET.2006.06.011
Dong Ju Son, Sandeep Kumar, Wakako Takabe, Chan Woo Kim, Chih-Wen Ni, Noah Alberts-Grill, In-Hwan Jang, Sangok Kim, Wankyu Kim, Sang Won Kang, Andrew H. Baker, Jai Woong Seo, Katherine W. Ferrara, Hanjoong Jo, The atypical mechanosensitive microRNA-712 derived from pre-ribosomal RNA induces endothelial inflammation and atherosclerosis Nature Communications. ,vol. 4, pp. 3000- 3000 ,(2013) , 10.1038/NCOMMS4000
Oliver Krämer, Sandra Klausing, Thomas Noll, Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches Applied Microbiology and Biotechnology. ,vol. 88, pp. 425- 436 ,(2010) , 10.1007/S00253-010-2798-6
Dethardt Müller, Hermann Katinger, Johannes Grillari, MicroRNAs as targets for engineering of CHO cell factories Trends in Biotechnology. ,vol. 26, pp. 359- 365 ,(2008) , 10.1016/J.TIBTECH.2008.03.010
Alice Gerrits, Marta A. Walasek, Sandra Olthof, Ellen Weersing, Martha Ritsema, Erik Zwart, Ronald van Os, Leonid V. Bystrykh, Gerald de Haan, Genetic screen identifies microRNA cluster 99b/let-7e/125a as a regulator of primitive hematopoietic cells. Blood. ,vol. 119, pp. 377- 387 ,(2012) , 10.1182/BLOOD-2011-01-331686
Vaibhav Jadhav, Matthias Hackl, Gerald Klanert, Juan A Hernandez Bort, Renate Kunert, Johannes Grillari, Nicole Borth, None, Stable overexpression of miR-17 enhances recombinant protein production of CHO cells. Journal of Biotechnology. ,vol. 175, pp. 38- 44 ,(2014) , 10.1016/J.JBIOTEC.2014.01.032