Phase-fluorimetry study on dielectric relaxation of human serum albumin.
作者： Andrea Buzády , János Erostyák , Béla Somogyi
DOI: 10.1016/S0301-4622(00)00210-6
关键词:
摘要: The dielectric relaxation (DR) of human serum albumin (HSA) was studied by the method phase-fluorometry. protein environment single tryptophan in HSA shows a relatively low-speed DR sub-ns characteristic time. This can be measured as decaying red-shift time-resolved fluorescence emission spectra. details calculations time-emission matrices (TEM) and comparison to data reference solution N-acetyl-L-tryptophanamide (NATA) are also presented.
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sci-hub.se 参考文章(34)
J.R. Lakowicz, H. Cherek, Dipolar relaxation in proteins on the nanosecond timescale observed by wavelength-resolved phase fluorometry of tryptophan fluorescence. Journal of Biological Chemistry. ,vol. 255, pp. 831- 834 ,(1980) , 10.1016/S0021-9258(19)86104-5
Wim G. J. Hol, Louis M. Halie, Christian Sander, DIPOLES OF THE ALPHA-HELIX AND BETA-SHEET - THEIR ROLE IN PROTEIN FOLDING Nature. ,vol. 294, pp. 532- 536 ,(1981) , 10.1038/294532A0
K. J. Willis, A. G. Szabo, M. Zuker, J. M. Ridgeway, B. Alpert, Fluorescence decay kinetics of the tryptophyl residues of myoglobin: effect of heme ligation and evidence for discrete lifetime components Biochemistry. ,vol. 29, pp. 5270- 5275 ,(1990) , 10.1021/BI00474A008
Xiao Min He, Daniel C. Carter, Atomic structure and chemistry of human serum albumin. Nature. ,vol. 358, pp. 209- 215 ,(1992) , 10.1038/358209A0
Stephen J. Hagen, William A. Eaton, Nonexponential structural relaxations in proteins Journal of Chemical Physics. ,vol. 104, pp. 3395- 3398 ,(1996) , 10.1063/1.471044
Eira Laitinen, Keijo Salonen, Timo Harju, Solvation dynamics study of 4‐amino‐N‐methyl‐phthalimide in n‐alcohol solutions Journal of Chemical Physics. ,vol. 104, pp. 6138- 6148 ,(1996) , 10.1063/1.471279
K. Chu, R. M. Ernst, H. Frauenfelder, J. R. Mourant, G. U. Nienhaus, R. Philipp, Light-induced and thermal relaxation in a protein. Physical Review Letters. ,vol. 74, pp. 2607- 2610 ,(1995) , 10.1103/PHYSREVLETT.74.2607
Xueyu Song, David Chandler, R. A. Marcus, GAUSSIAN FIELD MODEL OF DIELECTRIC SOLVATION DYNAMICS The Journal of Physical Chemistry. ,vol. 100, pp. 11954- 11959 ,(1996) , 10.1021/JP960887E
Joseph R. Lakowicz, Henryk Cherek, Ignacy Gryczynski, Nanda Joshi, Michael L. Johnson, Analysis of fluorescence decay kinetics measured in the frequency domain using distributions of decay times Biophysical Chemistry. ,vol. 28, pp. 35- 50 ,(1987) , 10.1016/0301-4622(87)80073-X
Alexander P. Demchenko, Jacques Gallay, Michel Vincent, Hans-Juergen Apell, Fluorescence heterogeneity of tryptophans in Na,K-ATPase: evidences for temperature-dependent energy transfer. Biophysical Chemistry. ,vol. 72, pp. 265- 283 ,(1998) , 10.1016/S0301-4622(98)00107-0