Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris).

摘要: SUMMARY The northern elephant seal pup ( Mirounga angustirostris ) undergoes a 2–3 month post-weaning fast, during which it depends primarily on the oxidation of fatty acids to meet its energetic demands. concentration non-esterified (NEFAs) increases and is associated with development insulin resistance in late-fasted pups. Furthermore, plasma NEFA concentrations respond differentially an intravenous glucose tolerance test (ivGTT) depending fasting duration, suggesting that effects lipid metabolism are altered. However, elucidation lipolytic mechanisms including lipase activity prolonged mammals scarce. To assess impact regulation metabolism, adipose tissue samples were collected before after ivGTTs performed early (2 weeks, N =5) late (6–8 weeks; =8) fasted Glucose administration increased triglycerides seals, but not glycerol. Fasting decreased basal by 50%. also twofold expressions CD36, FAS, FATP1 PEPCK-C 22–43% tissue. Plasma acylcarnitine profiling indicated seals display higher incomplete LCFA β-oxidation. Results suggest long-term induces shifts lipolysis onset Delineation responsible for this shift can contribute more thorough understanding changes dyslipidemia mammals.