In vivo adenovirus-mediated p53 tumor suppressor gene therapy for colorectal cancer.

摘要: Background: The p53 tumor suppressor gene is altered in up to 70% of colorectal cancers. Materials and Methods: We infected the cancer cell lines SW620 KM12L4, which mutated, with replication-defective adenovirus Ad5/CMV/p53 evaluate effects adenovirus-mediated wild-type transfer. Gene transduction was measured by cytochemical staining cells Ad5/CMV/β-gal virus expression wildtype protein these demonstrated immunoblotting. Results: Significant suppression vitro proliferation induction apoptosis (as TUNEL assay labeling) were observed following infection. More importantly, similar vivo an established nude mouse subcutaneous model; significant growth (60% -70%) intratumoral injections Ad5/CMV/p53. Conclusion: This form therapy may provide a novel approach cancer.