C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development.

摘要: Cold, hyperosmolarity, and abscisic acid (ABA) signaling induce RD29A expression, which is an indicator of the plant stress adaptation response. Two nonallelic Arabidopsis thaliana (ecotype C24) T-DNA insertional mutations, cpl1 cpl3, were identified based on hyperinduction expression that was monitored by using luciferase (LUC) reporter gene (RD29A:LUC) imaging system. Genetic linkage analysis complementation data established recessive cpl3 mutations are caused insertions in AtCPL1 (Arabidopsis C-terminal domain phosphatase-like) AtCPL3, respectively. Gel assays recombinant AtCPL3 detected innate phosphatase activity like other members phylogenetically conserved family dephosphorylate RNA polymerase II (RNAP II). mutation causes RD29A:LUC hyperexpression transcript accumulation response to cold, ABA, NaCl treatments, whereas mediates hyperresponsiveness only ABA. Northern confirmed LUC also occurs these stimuli. plants accumulate biomass more rapidly exhibit delayed flowering relative wild type grow slowly flower earlier than wild-type plants. Hence negative regulators responsive transcription modulators growth development. These results suggest regulation RNAP phosphorylation status a focal control point complex processes responses AtCPL apparently have both unique overlapping transcriptional regulatory functions differentiate signal output determines