Presence of labeled monocytes, macrophages and microglia in a stab wound of the brain following an injection of bone marrow cells labeled with 3H‐uridine into rats

摘要: To clarify the origin of cells appearing in a stab wound parietal cortex, bone marrow from inbred Lewis rats were labeled vitro with 3H-uridine and injected intravenously into 36 same strain Just before or after brain had been stabbed. The animals sacrificed 1 to 15 days injection cells. examined percentage various types recorded. Radioautographs prepared surrounding neuropil, as well corresponding region on intact side; they searched for cells. One day stabbing, consisted central containing fluid red blood cells, marginal disorganized nervous tissue including granulocytes, monocytes macrophages. Around wound, neuropil appeared normal, except perivascular areas more cellular distended than normally. During next few days, granulocytes gradually disappeared wound. Meanwhile, macrophages, which initially often showed monocytic features, became increasingly numerous. However, seventh day, macrophages decreased number, while many small These displayed scanty basophilic cytoplasm rich phagosomes nucleus dense chromatin clumps similar that seen microglia A search radioautographs revealed no cell side, but 220 injured side. Most these within itself, neuropil. Within common one injection, first three days. Few observed at days; none however, possibly due turnover loss RNA. In identified microglia during injection. They located areas, satellite position neurons nearby subependymal layer It is concluded that, under influence enter presence features indicates transform displaying microglia-like nucleus, become numerous are tentatively interpreted intermediates transformation monocytederived microglia. Finally, early direct