Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes

摘要: The mechanisms by which the epithelium of digestive tract and its associated glands are specified largely unknown. One clue is that several transcription factors expressed in specific regions endoderm prior to during organogenesis. Pdx-1, for example, duodenum pancreas Pdx-1 inactivation results an arrest pancreatic development after buds formation. Similarly, ngn3 transiently developing a knockout absence endocrine cells. This paper focuses on question whether these other factors, known be necessary development, also sufficient drive program Using ovo electroporation chick embryos, we show ectopic expression or causes cells bud out like progenitors. Pdx-1-expressing extinguish markers nonpancreatic initiate, but do not complete, cytodifferentiation. Ectopic turn endodermal any region into form islets expressing glucagon somatostatin mesenchyme. suggest simple gene combinations could used stem achieve tissue differentiation.