IL-27 affects helper T cell responses via regulation of PGE2 production by macrophages

摘要: IL-27 is a heterodimeric cytokine that regulates both innate and adaptive immunity. The immunosuppressive effect of largely depends on induction IL-10-producing Tr1 cells. To date, however, effects regulation immune responses via mediators other than cytokines remain poorly understood. address this issue, we examined immunoregulatory conditional medium bone marrow-derived macrophages (BMDMs) from WSX-1 (IL-27Rα)-deficient mice found enhanced IFN-γ IL-17A secretion by CD4(+) T cells as compared with control BMDMs. We then PGE2 production COX-2 expression BMDMs WSX-1-deficient was increased to in response LPS. abolished EP2 EP4 antagonists, demonstrating responsible for production. Murine WSX-1-expressing Raw264.7 (mWSX-1-Raw264.7) showed phosphorylation STAT1 STAT3 produced less amounts parental RAW264.7 knockdown STAT1-deficiency higher their respective Collectively, our result indicated IL-27/WSX-1 regulated STAT1-COX-2 pathway affected helper cell PGE2-mediated fashion.