A low-toxic and efficient gene vector: carboxymethyl dextran-graft-polyethylenimine.
作者： Yun-Xia Sun , Xian-Zheng Zhang , Han Cheng , Si-Xue Cheng , Ren-Xi Zhuo
DOI: 10.1002/JBM.A.31520
关键词:
摘要: In this study, the low molecular weight branched polyethylenimine (PEI) (800 Da PEI) was grafted to biodegradable and biocompatible carboxymethyl dextran (CMD) obtain CMD-g-PEI, plasmid DNA complexed with CMD-g-PEI polycation form polyion complex. The acid-base titration profile showed that had endosomal disruption capacity, agarose gel electrophoresis suggested could condense efficiently. transfection efficiency of CMD-g-PEI/DNA complexes measured by luciferase green fluorescent protein assay in HEK293 cells. results revealed at N/P ratios over 30–70 higher than or comparable 25 kDa PEI optimal ratio (N/P = 10). cytotoxicity as well evaluated NIH3T3 cells, respectively, it also found much lower PEI. resulted high have promising applications when used gene vector. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
sci-hub.se 参考文章(25)
Joon Sig Choi, Eun Jung Lee, Hyung Suk Jang, Jong Sang Park, New Cationic Liposomes for Gene Transfer into Mammalian Cells with High Efficiency and Low Toxicity Bioconjugate Chemistry. ,vol. 12, pp. 108- 113 ,(2001) , 10.1021/BC000081O
Farzaneh Fayazpour, Bart Lucas, Carmen Alvarez-Lorenzo, Niek N. Sanders, Jo Demeester, Stefaan C. De Smedt, Physicochemical and Transfection Properties of Cationic Hydroxyethylcellulose/DNA Nanoparticles Biomacromolecules. ,vol. 7, pp. 2856- 2862 ,(2006) , 10.1021/BM060474B
Wu, Ye Liu, Xuan Jiang, He, Suat Hong Goh, Kam W. Leong, Hyperbranched Poly(amino ester)s with Different Terminal Amine Groups for DNA Delivery Biomacromolecules. ,vol. 7, pp. 1879- 1883 ,(2006) , 10.1021/BM0601878
Jonathan M. Benns, Joon-Sig Choi, Ram I. Mahato, Jong-Sang Park, Sung Wan Kim, pH-sensitive cationic polymer gene delivery vehicle: N-Ac-poly(L-histidine)-graft-poly(L-lysine) comb shaped polymer. Bioconjugate Chemistry. ,vol. 11, pp. 637- 645 ,(2000) , 10.1021/BC0000177
Kang Moo Huh, Junko Hashi, Toor Ooya, Nobuhiko Yui, Synthesis and characterization of dextran grafted with poly(N-isopropylacrylamide-co-N,N-dimethyl-acrylamide) Macromolecular Chemistry and Physics. ,vol. 201, pp. 613- 619 ,(2000) , 10.1002/(SICI)1521-3935(20000301)201:5<613::AID-MACP613>3.0.CO;2-I
Youxiang Wang, Ping Chen, Jiacong Shen, The development and characterization of a glutathione-sensitive cross-linked polyethylenimine gene vector Biomaterials. ,vol. 27, pp. 5292- 5298 ,(2006) , 10.1016/J.BIOMATERIALS.2006.05.049
Qing Cai, Yuqing Wan, Jianzhong Bei, Shenguo Wang, Synthesis and characterization of biodegradable polylactide-grafted dextran and its application as compatilizer Biomaterials. ,vol. 24, pp. 3555- 3562 ,(2003) , 10.1016/S0142-9612(03)00199-6
Tony Azzam, Arthur Raskin, Arik Makovitzki, Henry Brem, Pierre Vierling, Michal Lineal, Abraham J Domb, None, Cationic Polysaccharides for Gene Delivery Macromolecules. ,vol. 35, pp. 9947- 9953 ,(2002) , 10.1021/MA0209592
Tony Azzam, Hagit Eliyahu, Libi Shapira, Michal Linial, Yechezkel Barenholz, Abraham J Domb, None, Polysaccharide-oligoamine based conjugates for gene delivery. Journal of Medicinal Chemistry. ,vol. 45, pp. 1817- 1824 ,(2002) , 10.1021/JM0105528
Yoshikazu Kumashiro, Kang Moo Huh, Tooru Ooya, Nobuhiko Yui, Modulatory factors on temperature-synchronized degradation of dextran grafted with thermoresponsive polymers and their hydrogels. Biomacromolecules. ,vol. 2, pp. 874- 879 ,(2001) , 10.1021/BM015527Y