Epidemiological typing of meticillin-resistant Staphylococcus aureus isolates from Pakistan and India.

摘要: The levels of meticillin-resistant Staphylococcus aureus (MRSA) in Pakistan and India are known to be high, but few studies have described the epidemiology different MRSA clones present. In order gain an understanding within this region, 60 isolates from (49) (11) were genotyped. All typed using PFGE, staphylococcal interspersed repeat units (SIRUs), a restriction-modification method cassette chromosome mec (SCCmec) typing. A subset that distinct by PFGE SIRUs multilocus sequence typing (MLST). Clonal complex (CC) 8 was dominant clonal (57/60) present both India. Within CC8, there 10 SIRU profiles 24 profiles. Two Pakistan, whilst seven for one each two countries. Thirty-four 57 carried SCCmec type III/IIIa remainder IV SCCmec. MLST analysis 14 CC8 with diverse showed all single-locus variants, nine belonging (ST) 239, three ST8 ST113. From single hospital belonged CC30 indistinguishable Panton-Valentine leukocidin gene. Thus, epidemiological strains locations revealed predominance highly related STs. ability differentiate ST239 demonstrates their utility defining local these