Effect of ERCC-1 overexpression on sensitivity of Chinese hamster ovary cells to DNA damaging agents.

摘要: Previous studies of ERCC-1 gene expression levels in chronic lymphocytic leukemia and ovarian carcinoma tumor specimens indicated that increased may correlate with a lack response to the alkylating agents, melphalan, cis -diamminedichloroplatinum (II). In order demonstrate direct involvement protein repair melphalan lesions, defective Chinese hamster ovary cell line UV20 was transfected human complementary DNA. Stably cells demonstrated an increase resistance melphalan. Wild type AA8 were then stably same The result sensitivity There no effect on uv light, but had These results suggest overexpression inhibit pathway specific bifunctional DNA damaging agent lesions cells. should be useful determining precise role cross-links induced by