Phloretin inhibits interleukin-1β-induced COX-2 and ICAM-1 expression through inhibition of MAPK, Akt, and NF-κB signaling in human lung epithelial cells.

摘要: Phloretin, a flavonoid isolated from the apple tree, is reported to have anti-inflammatory, anti-oxidant, and anti-adiposity effects. In this study, we evaluated suppressive effects of phloretin on intercellular adhesion molecule 1 (ICAM-1) cyclooxygenase (COX)-2 expression in IL-1β-stimulated human lung epithelial A549 cells. The cells were pretreated with various concentrations (3–100 μM), followed by induced inflammation IL-1β. Phloretin inhibited levels prostaglandin E2, decreased COX-2 expression, suppressed IL-8, monocyte chemotactic protein 1, IL-6 production. It also ICAM-1 gene inflammatory significantly Akt mitogen-activated kinase (MAPK) phosphorylation nuclear transcription factor kappa-B (NF-κB) subunit p65 translocation into nucleus. addition, was pretreatment both MAPK inhibitors However, phlorizin, derivative phloretin, did not suppress response These results suggest that might an anti-inflammatory effect inhibiting proinflammatory cytokine, COX-2, via blocked NF-κB signaling pathways.