Expression of porcine parvovirus VP2 gene requires codon optimized E. coli cells
作者： Ting Qi , Shangjin Cui
DOI: 10.1007/S11262-009-0378-6
关键词:
摘要: Porcine parvovirus (PPV) is a widespread infectious virus that causes serious reproductive diseases of swine and death piglets. The gene coding for the capsid protein VP2 PPV was amplified inserted into plasmid pET-32a (+), which then used to transform Escherichia coli Rosetta, fused polyhistidine tag, position affinity tag in N-terminus. expressed using different expression host bacteria, including E. BL21, vectors, pET-30a pGEX-6p-1. After selection, only fusion (+) well Rosetta. recombinant bacterium produced high quantities VP2, about 8% total. antigenically similar native according Western blot assay performed with polyclonal antibodies obtained from pigs vaccinated PPV. A simple, easily commercialized procedure purify this protein. This study provides foundation application clinical diagnosis vaccination against
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