Expression of chicken parvovirus VP2 in chicken embryo fibroblasts requires codon optimization for production of naked DNA and vectored meleagrid herpesvirus type 1 vaccines.

摘要: Meleagrid herpesvirus type 1 (MeHV-1) is an ideal vector for the expression of antigens from pathogenic avian organisms in order to generate vaccines. Chicken parvovirus (ChPV) a widespread infectious virus that causes serious disease chickens. It one etiological agents largely suspected causing Runting Stunting Syndrome (RSS) Initial attempts express wild-type gene encoding capsid protein VP2 ChPV by insertion into thymidine kinase MeHV-1 were unsuccessful. However, transient codon-optimized synthetic cloned bicistronic pIRES2-Ds-Red2, could be demonstrated immunocytochemical staining transfected chicken embryo fibroblasts (CEFs). Red fluorescence also detected these cells since red fluorescent downstream internal ribosome entry site (IRES). Strikingly, not transiently with containing or non-codon-optimized gene. Immunocytochemical failed demonstrate VP2, indicating lack was at RNA level and toxic CEFs. Chickens vaccinated DNA vaccine consisting elicited humoral immune response as measured VP2-specific ELISA. This cassette rescued genome generating vectored against disease.