A Microassay for the detection of low levels of cytochrome P450 O-deethylation activities with alkoxyresorufin substrates
作者： Jean Lagueux , El Bachir Affar , Denis Nadeau , Pierre Ayotte , Éric Dewailly
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摘要: A microfluorometric method for the detection of low levels cytochrome P450 was developed to increase sensitivity assay, since a level CYP450 associated enzymatic activities expected in human placenta tissues and small samples (∼10 g) could be easily collected, stored processed. The dual fluorescence assay Kennedy et al. [1], which simultaneously quantitate microsomal proteins ethoxyresorufin-O-deethylase (EROD) activity adapted 96 wells microtiter plates. Placental microsomes were analyzed. For obtained from non-smoking mothers general southern Quebec population, results ranged less than 1–3.3 pmol/mg protein•min. Samples collected smoking showed ranging 30–69 These suitability microassay measuring such as placenta. (Mol Cell Biochem 175: 131–136, 1997)
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sci-hub.se 参考文章(15)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Walter G. BALVERS, Marelle G. BOERSMA, Cees VEEGER, Ivonne M. C. M. RIETJENS, Kinetics of cytochromes P‐450 IA1 and IIB1 in reconstituted systems with dilauroyl‐ and distearoyl‐glycerophosphocholine FEBS Journal. ,vol. 215, pp. 373- 381 ,(1993) , 10.1111/J.1432-1033.1993.TB18043.X
Roberta J. Pohl, James R. Fouts, A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions Analytical Biochemistry. ,vol. 107, pp. 150- 155 ,(1980) , 10.1016/0003-2697(80)90505-9
M.T. Donato, M.J. Gomezlechon, J.V. Castell, A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates Analytical Biochemistry. ,vol. 213, pp. 29- 33 ,(1993) , 10.1006/ABIO.1993.1381
M.Danny Burke, Stephanie Thompson, Clifford R. Elcombe, James Halpert, Tapio Haaparanta, Richard T. Mayer, Ethoxy-, pentoxy- and benzyloxyphenoxazones and homologues: a series of substrates to distinguish between different induced cytochromes P-450. Biochemical Pharmacology. ,vol. 34, pp. 3337- 3345 ,(1985) , 10.1016/0006-2952(85)90355-7
David K Manchester, Elizabeth H Jacoby, Sensitivity of human placental monooxygenase activity to maternal smoking Clinical Pharmacology and Therapeutics. ,vol. 30, pp. 687- 692 ,(1981) , 10.1038/CLPT.1981.221
Allan B. Okey, Enzyme induction in the cytochrome P-450 system Pharmacology & Therapeutics. ,vol. 45, pp. 241- 298 ,(1990) , 10.1016/0163-7258(90)90030-6
M.D. Burke, S. Orrenius, Isolation and comparison of endoplasmic reticulum membranes and their mixed function oxidase activities from mammalian extrahepatic tissues. Pharmacology & Therapeutics. ,vol. 7, pp. 549- 599 ,(1979) , 10.1016/0163-7258(79)90042-1
Markku Pasanen, Olavi Pelkonen, Xenobiotic and steroid-metabolizing monooxygenases catalysed by cytochrome P450 and glutathione S-transferase conjugations in the human placenta and their relationships to maternal cigarette smoking. Placenta. ,vol. 11, pp. 75- 85 ,(1990) , 10.1016/S0143-4004(05)80445-X
S.W. Kennedy, S.P. Jones, Simultaneous Measurement of Cytochrome P4501A Catalytic Activity and Total Protein Concentration with a Fluorescence Plate Reader Analytical Biochemistry. ,vol. 222, pp. 217- 223 ,(1994) , 10.1006/ABIO.1994.1476