A method for direct soil extraction and PCR amplification of endomycorrhizal fungal DNA
作者： V. P. Claassen , R. J. Zasoski , B. M. Tyler
关键词:
摘要: DNA from endomycorrhizal fungi was extracted directly a weathered soil (alfisol) mixed with sand. Mycorrhizae were established in greenhouse culture of Glomus clarum Sudan grass (Sorghum vulgare var. sudanense) host plants. The extraction procedure included enzymatic digestion cell walls, sodium dodecyl sulfate lysis cells, polyvinylpolypyrrolidone absorption organic compounds, and ethanol precipitation the DNA. extracts amplified by polymerase chain reaction using primers nuclear 17S rRNA sequence that general to or specific endomycorrhizae.
springer.com
sci-hub.se 参考文章(22)
Paul T. Rygiewicz, John L. Armstrong, 9 Ectomycorrhizal DNA: Isolation, RFLPs and Probe Hybridization Methods in Microbiology. ,vol. 23, pp. 253- 280 ,(1991) , 10.1016/S0580-9517(08)70179-X
William E. Holben, Janet K. Jansson, Barry K. Chelm, James M. Tiedje, DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community Applied and Environmental Microbiology. ,vol. 54, pp. 703- 711 ,(1988) , 10.1128/AEM.54.3.703-711.1988
T Ersek, J E Schoelz, J T English, PCR amplification of species-specific DNA sequences can distinguish among Phytophthora species. Applied and Environmental Microbiology. ,vol. 60, pp. 2616- 2621 ,(1994) , 10.1128/AEM.60.7.2616-2621.1994
R J Steffan, J Goksøyr, A K Bej, R M Atlas, Recovery of DNA from soils and sediments. Applied and Environmental Microbiology. ,vol. 54, pp. 2908- 2915 ,(1988) , 10.1128/AEM.54.12.2908-2915.1988
L Simon, M Lalonde, T D Bruns, Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Applied and Environmental Microbiology. ,vol. 58, pp. 291- 295 ,(1992) , 10.1128/AEM.58.1.291-295.1992
S D Pillai, K L Josephson, R L Bailey, C P Gerba, I L Pepper, Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences Applied and Environmental Microbiology. ,vol. 57, pp. 2283- 2286 ,(1991) , 10.1128/AEM.57.8.2283-2286.1991
Richard T. D'Aquila, Lawrence J. Bechtel, Joseph A. Videler, Joseph J. Eron, Paul Gorczyca, Joan C. Kaplan, Maximizing sensitivity and specificity of PCR by preamplification heating Nucleic Acids Research. ,vol. 19, pp. 3749- 3749 ,(1991) , 10.1093/NAR/19.13.3749
Alejandro Viera, Marian G. Glenn, DNA content of vesicular-arbuscular mycorrhizal fungal spores. Mycologia. ,vol. 82, pp. 263- 267 ,(1990) , 10.2307/3759857
Andrew Ogram, Gary S. Sayler, Tamar Barkay, The extraction and purification of microbial DNA from sediments Journal of Microbiological Methods. ,vol. 7, pp. 57- 66 ,(1987) , 10.1016/0167-7012(87)90025-X
Agnete Fægri, Vigdis Lid Torsvik, J. GoksÖyr, Bacterial and fungal activities in soil: Separation of bacteria and fungi by a rapid fractionated centrifugation technique Soil Biology and Biochemistry. ,vol. 9, pp. 105- 112 ,(1977) , 10.1016/0038-0717(77)90045-1