The application of bovine brain microvessel endothelial-cell monolayers grown onto polycarbonate membranes in vitro to estimate the potential permeability of solutes through the blood-brain barrier.

摘要: Previously our laboratory (Rim et al., Int. J. Pharm. 32:79-84. 1986) described an in vitro blood-brain barrier (BBB) model consisting of cultured bovine brain microvessel endothelial cells (BMECs) grown onto regenerated cellulose acetate membranes. However, the utility this BBB system was limited because membrane and not monolayer BMECs rate limiting for permeability very lipophilic compounds. Therefore, study we have evaluated polycarbonate membranes as supports growing conducting drug studies. Bovine were on collagen-coated (13-mm diameter, 12-microns pore size) which then mounted into side-by-side diffusion transport The permeabilities a series solutes varying lipophilicity (progesterone, estrone, testosterone, haloperidol, propranolol, antipyrine, caffeine, urea, acyclovir, ganciclovir, ribavirin, glycerol) determined excellent correlation (r = 0.97) established between coefficients their log partition (PC)/(MW)1/2. These results suggest that can be used estimating potential solute through vivo.