Intraperitoneal Photoimmunotherapy of Ovarian Carcinoma Xenografts in Nude Mice Using Charged Photoimmunoconjugates

摘要: Abstract Objective. The objective of this study was to compare the efficacy photoimmunoconjugates with cationic and anionic molecular charges on intraperitoneal photoimmunotherapy ovarian cancer xenografts in nude mice. Methods. photosensitizer chlorin e6 (c ) conjugated via a poly-l-lysine linker F(ab′) 2 fragment murine anti-ovarian monoclonal antibody OC125, resulting photoimmunoconjugate pronounced charge. Alternatively, by succinylating conjugate, charge obtained. A model derived from inoculation NIH:OVCAR-5 cells employed. conjugate injected intraperitoneally followed after 3 h red light delivered through fiber into peritoneal cavity. These treatments were repeated three times, results obtained compared those free c control. extent residual macroscopic disease death evaluable outcomes for tumoricidal survival studies, respectively. Results. In contrast other photosensitizers, mice showed no systemic toxicity or morbidity treatment. initial mean tumor weights all treatment groups ranged 33 73 mg, as 330 mg untreated controls ( P = 0.009). Conclusion. Photoimmunotherapy produces superior an further optimization regimen may lead potential advanced cancer.