msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation

摘要: Abstract Messenger RNA transcripts of the highly pigmented murine melanoma B16-F1 cells were compared with those from their weakly derivative B16-F10 by differential display. A novel gene called msg1 (melanocyte-specific gene) was found to be expressed at high levels in but low cells. Expression undetectable amelanotic K1735 The melanocyte cell line melan-a msg1, as did primary cultures and human melanocytes; however, seven or very lines negative. Transformation melanocytes transfection v-Ha-ras Ela accompanied depigmentation led complete loss expression. normal tissue distribution mRNA adult mice confined testis. Murine MSG1 genes encode a predicted protein 27 kDa 75% overall amino acid identity 96% within C-terminal acidic domain 54 acids. This conserved 76% another product gene, MRG1 (msg1-related gene), isolated cDNA 5'-rapid amplification ends based on homology msg1. localized nucleus immunofluorescence cytochemistry. We conclude that encodes nuclear protein, is melanocyte-specific, appears lost depigmented