Vitamin E-loaded dialyzer resets PBMC-operated cytokine network in dialysis patients.

摘要: Vitamin E–loaded dialyzer resets PBMC-operated cytokine network in dialysis patients. Background In hemodialysis patients the activity of stimulated Th1 lymphocytes is depressed, while Th2 cells are constitutively primed. Such phenomena may depend on monocyte activation and altered release interleukin (IL)-12 IL-18, which regulate Th cell differentiation. Reactive oxygen species (ROS) activate monocytes; therefore, a hemodialyzer with antioxidant would contrast ROS, prevent activation, reset IL-12 IL-18 release, restore Th1/Th2 balance. Methods Ten regular treatment (RDT) cellulosic membrane (CM) were shifted to vitamin E–coated (VE). During CM after 3, 6, 12months VE, peripheral blood mononuclear (PBMC) purified CD4+ isolated, cultured, resting, mitogen-stimulated, interferon γ (IFNγ), IL-4, IL-10, IL-12, was measured. E A plasma levels effects single session NO assayed. Results The constitutive IL-4 IL-10 by abated significantly VE (nadir -77.8% -55.3%, respectively, at 12months). INFγ mitogen-stimulated recovered (zenith +501% PBMC production reduced -64.7% -51.3%, respectively). increased vitamins A. fell (-17%, P Conclusion cytokines released monocytes toward normality dialyzer.