Performance evaluation of four 25-hydroxyvitamin D assays to measure 25-hydroxyvitamin D2.

摘要: Abstract Objectives The ability of current immunoassays to accurately measure equimolar amounts 25(OH)D 2 and 3 has been recently questioned. This study determined serum , total concentrations in healthy vitamin D -supplemented subjects by isotope dilution liquid chromatography mass spectrometry (ID-LC-MS/MS); and, evaluated the Siemens, DiaSorin, Roche, Abbott Vitamin Total assays monitor compared an ID-LC-MS/MS method traceable National Institute Standards Technology (NIST), that achieved certification from Centers for Disease Control Prevention (CDC) Standardization Certification Program (VDSCP). Design methods Twenty (20) adults, with no history prior supplementation were administered oral (2400 IU/day 6 months). Serum samples (140) baseline monthly blood draws tested. Results After one month, mean rose 0.8 43.6 nmol/L, whereas declined 84.0 63.4 nmol/L; 86.6 107.0 nmol/L. overall bias was − 7.1% Siemens ADVIA Centaur assay, − 15.3% DiaSorin LIAISON assay; − 8.4% Roche ELECSYS assay − 16.3% ARCHITECT assay. Correlation coefficients (r) 0.94, 0.79, 0.74, 0.73; [25(OH) -containing] versus six-month [25(OH)D - − 13.4% − 5.7%; − 3.5% 20.3%, 9.6% − 12.1%, 0.2% − 17.8%, respectively. Conclusions results obtained slightly lower than those but all demonstrated acceptable performance.