Mechanisms of IFN-gamma induction by natural killer cell stimulatory factor (NKSF/IL-12). Role of transcription and mRNA stability in the synergistic interaction between NKSF and IL-2.

摘要: We have investigated the molecular mechanisms regulating IFN-gamma production in human T lymphocytes stimulated by NK cell stimulatory factor (NKSF/IL-12). show that NKSF synergizes with IL-2 and phorbol diesters inducing accumulation of mRNA PHA-activated blasts. regulates expression PHA blasts leukemia line, TALL-103/2, at both transcriptional posttranscriptional levels. increases rate for these types, as determined nuclear run-on analysis. However, synergy between can be demonstrated only level stability, cytokines are required to increase half-life.