Hydrolysis and transformation of cellulose with Aspergillus niger IBT-90 enzymes

摘要: Hydrolysis and transformation of Fibrenier cellulose (USA) with enzymes from Aspergillus niger IBT-90 was studied. The process performed at 50 °C pH 4.8 for 24 h using an enzyme complex either as a properly diluted culture filtrate or mixture isolated purified A. IBT-90. In the latter experiments, enzyme-substrate ratios expressed units activity per 1 g were follows: endoglucanase E 2 , 40; β-glucosidase, 40 cellobiohydrolase, 2. Cellulose concentration 5%. It proved that crude cellulolytic IBT-9() exhibits higher efficiency in decomposition comparison to this complex, revealed assays reducing sugars determinations light transmission throughout fibres computer analysis microscopic image. Comparison both endoglucanases showed first is more active against cellulose. liberated caused significant fibres. predominant effect smoothing fibre surface. cellobiohydrolase split into many short