Rotavirus genotypes in children in the community with diarrhea in Madagascar.

摘要: In the context of possible introduction a preventive vaccine against rotaviruses in Madagascar, G and P genotypes distribution circulating children Madagascar was studied, presence emerging unusual strains were assessed. From February 2008 to May 2009, 1,679 stools specimens collected from ≤5 years old with diarrhea. ELISA used for antigen detection, molecular amplification VP7 VP4 gene fragments genotyping. Rotavirus detected 104 samples (6.2%). Partial sequences genes obtained 81 80 antigen-positive stools, respectively. The most frequent types combinations G9P[8] (n = 51; 64.6%), followed by G1P[8] (n = 15; 18.9%), G1P[6] (n = 8; 10.1%). A few G-P combinations, such as G4P[6] (n = 3; 3.8%), G9P[6] (n = 1; 1.3%), G3P[9] reassortant feline human virus 1.3%) identified. Both one three isolates closely related those porcine strains, virus. These findings give an overview should help public health authorities define strategy.