Epigallocatechin gallate protects against nitric oxide-induced apoptosis via scavenging ROS and modulating the Bcl-2 family in human dental pulp cells.

摘要: Nitric oxide (NO) is produced by three different isoforms of the enzyme NO synthase (NOS). NOS are expressed in many cell types, including human dental pulp cells (HDPC). acts as an intracellular messenger at physiological levels although it can be cytotoxic higher concentrations. Epigallocatechin gallate (EGCG), a major green tea polyphenol, has diverse pharmacological activities growth and death. This study aimed to investigate apoptotic mechanism effects EGCG on NO-induced apoptosis HDPC. Sodium nitroprusside (SNP), donor, decreased viability HDPC dose- time-dependent manner. was administered for 1 hr before SNP treatment, resulting increased reactive oxygen species (ROS) production inhibition. Expression Bax, pro-apoptotic Bcl-2 family, upregulated, whereas expression Bcl-2, anti-apoptotic downregulated SNP-treated augmented release cytochrome c from mitochondria into cytosol enhanced caspase-9, -3 activities, marker executing stage. ameliorated caspase-9 SNP. These results suggest that protective effect against scavenging ROS modulating family.