Androgen and retinoic acid interaction in LNCaP cells, effects on cell proliferation and expression of retinoic acid receptors and epidermal growth factor receptor

摘要: Modulation of the expression retinoic acid receptors (RAR) α and γ in adult rat prostate by testosterone (T) suggests that RAR signaling events might mediate some androgen effects on cells. In this study, we examined interactions between T (RA) cell growth human carcinoma cells, LNCaP, their relationship with epidermal factor receptor (EGF-R). Both RA, when administered alone, stimulated 3H-thymidine incorporation LNCaP cells a dose-dependent manner; effect each agent was reciprocally attenuated other agent. Testosterone treatment also resulted dose dependent, biphasic increases mRNAs; paralleled were presence 100 nM RA. These results suggest link growth. Gel electrophoretic mobility shift assays revealed putative responsive element (ARE) promoter region gene, suggesting direct AR-DNA interaction gene. Furthermore, 20 an increase EGF-R. contrast, EGF-R suppressed RA T. Current demonstrate RARs The ARE gene opposite signal these be involved control