Cellular dynamics of corneal wound re-epithelialization in the rat. I. Fate of ocular surface epithelial cells synthesizing DNA prior to wounding

摘要: The fate of ocular surface epithelial cells in response to injury the cornea was examined. Corneal were labeled during DNA synthesis with [3H]thymidine 1 h prior wounding. A 3-mm diameter defect made center rat cornea, basement membrane remaining intact. Within 12 abrasion, detected regenerating surface. At 18 h, there a 2.7- and 17-fold increase basal suprabasal cells, respectively, epithelium adjacent wound, at 24 30 an excessive number cell layers (up 7) margin abrasion. Re-epithelialization progressed as gradient that became diminished towards wound. Completion 1, 2, 3, 4 recorded 24, 30, 36, 72 respectively. No changes labeling index limbus or conjunctiva noted. These results suggest processes centripetal vertical migration, well events related division, uninjured corneal are not impeded by wounding epithelium. However, wound healing appears require phenotype, presumably because this type's migratory capability.