Detection of Listeria monocytogenes in pork and beef using the VIDAS® LMO2 automated enzyme linked immunoassay method.

作者: Cornelia Meyer , Maria Fredriksson-Ahomaa , Brigitte Sperner , Erwin Märtlbauer

DOI: 10.1016/J.MEATSCI.2011.01.035

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摘要: Abstract Listeria (L.) monocytogenes, a foodborne pathogen, is known to be possible contaminant of foods during production and processing. Samples (n = 985) raw meat by-products obtained from beef pork were first screened by the VIDAS system for presence spp., followed testing L. monocytogenes. Positive monocytogenes results confirmed plating on selective agars: 14% samples positive 4% tested which 3% agars. In (17%) contamination with listeriae was higher than in cuts (10%). Only strongly spp. Overall, prevalence rather low comparison most previous studies. The shown suitable method screening out Listeria-negative samples; main advantage being markedly reduced assay time.

参考文章(18)
Josef Kleer, Wondwossen A Gebreyes, Goetz Hildebrandt, Bayleyegn Molla Zewde, Marie-Thérèse Toquin, Desalegn Mengesha, Occurrence and distribution of Listeria monocytogenes and other Listeria species in ready-to-eat and raw meat products Berliner Und Munchener Tierarztliche Wochenschrift. ,vol. 122, pp. 20- 24 ,(2009)
TIINA AUTIO, ANNUKKA MARKKULA, SANNA HELLSTRÖM, TAINA NISKANEN, JANNE LUNDÉN, HANNU KORKEALA, Prevalence and genetic diversity of Listeria monocytogenes in the tonsils of pigs. Journal of Food Protection. ,vol. 67, pp. 805- 808 ,(2004) , 10.4315/0362-028X-67.4.805
Niels Skovgaard, Carl-Aage Morgen, Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin. International Journal of Food Microbiology. ,vol. 6, pp. 229- 242 ,(1988) , 10.1016/0168-1605(88)90015-3
A.I. Vitas, V.Aguado e I. Garcia-Jalon, Occurrence of Listeria monocytogenes in fresh and processed foods in Navarra (Spain) International Journal of Food Microbiology. ,vol. 90, pp. 349- 356 ,(2004) , 10.1016/S0168-1605(03)00314-3
Paolo Aureli, Giovanni Carlo Fiorucci, Daniela Caroli, Giovanna Marchiaro, Oreste Novara, Leonello Leone, Stefania Salmaso, An Outbreak of Febrile Gastroenteritis Associated with Corn Contaminated by Listeria monocytogenes The New England Journal of Medicine. ,vol. 342, pp. 1236- 1241 ,(2000) , 10.1056/NEJM200004273421702
Domenico Meloni, Pietro Galluzzo, Anna Mureddu, Francesca Piras, Mansel Griffiths, Rina Mazzette, Listeria monocytogenes in RTE foods marketed in Italy: Prevalence and automated EcoRI ribotyping of the isolates International Journal of Food Microbiology. ,vol. 129, pp. 166- 173 ,(2009) , 10.1016/J.IJFOODMICRO.2008.11.014
Tomoko TAKAHASHI, Yoshitsugu OCHIAI, Hiroki MATSUDATE, Kazuhiro HASEGAWA, Toshio SEGAWA, Masafumi FUKUDA, Ryo HONDO, Fukiko UEDA, Isolation of Listeria monocytogenes from the skin of slaughtered beef cattle. Journal of Veterinary Medical Science. ,vol. 69, pp. 1077- 1079 ,(2007) , 10.1292/JVMS.69.1077
Alasdair P. MacGowan, Karen Bowker, James McLauchlin, Peter M. Bennett, David S. Reeves, The occurrence and seasonal changes in the isolation of Listeria spp. in shop bought food stuffs, human faeces, sewage and soil from urban sources International Journal of Food Microbiology. ,vol. 21, pp. 325- 334 ,(1994) , 10.1016/0168-1605(94)90062-0
Takashi IIDA, Masako KANZAKI, Akiko NAKAMA, Yataro KOKUBO, Tsutomu MARUYAMA, Choji KANEUCHI, Detection of Listeria monocytogenes in humans, animals and foods. Journal of Veterinary Medical Science. ,vol. 60, pp. 1341- 1343 ,(1998) , 10.1292/JVMS.60.1341