Antioxidant and anti-inflammatory effects of piperine on UV-B-irradiated human HaCaT keratinocyte cells.

摘要: Abstract The increase in intracellular reactive oxygen and nitrogen species plays a key role ultraviolet B (UV-B)-induced inflammatory responses the human skin. Piperine exhibits many pharmacological benefits. In present study, photoprotective effects possible underlying mechanisms of anti-inflammatory piperine on UV-B-irradiated keratinocytes were investigated. exerted strong, direct scavenging DPPH radicals exhibited free radical capabilities as demonstrated by DCFH-DA Griess assays. Consistent with these results, 10, 20, 40 μM pretreatments attenuated UV-B irradiation-induced keratinocyte cytotoxicity reported resazurin assay. highest concentration inhibited cell apoptosis, revealed Hoechst 33342 staining. Moreover, we using western blot analysis, real-time PCR, ELISA. Pretreatment suppressed activation phosphorylated p38, JNK, AP-1 well levels COX-2/PGE2 iNOS synthesis, while cells triggered induction signaling molecules. These results indicated that inhibition pathways might play regulation piperine. addition, showed stronger than celecoxib which served positive control at same concentration. All suggested properties protected from UV-B-induced damage, be due to its antioxidant properties. Therefore, may an effective therapeutic candidate compound for treatment UV skin inflammation.