A tethered bilayer sensor containing alamethicin channels and its detection of amiloride based inhibitors

摘要: Alamethicin, a small transmembrane peptide, inserts into tethered bilayer membrane (tBLM) to form ion channels, which we have investigated using electrical impedance spectroscopy. The number of channels formed is dependent on the incubation time, concentration alamethicin and application DC voltage. properties when in bilayers are similar those for such assembled black lipid membranes (BLMs). Furthermore, amiloride certain analogs can inhibit channel pores, tBLMs. potency inhibitors be determined by measuring change impedance. Our work illustrates possibility synthetic tBLM study peptide voltage channels. A potential device as screening tool drug discovery processes.