Comparison of bioassays using the anostracan crustaceans Artemia salina and Thamnocephalus platyurus for plant extract toxicity screening

摘要: Three lethality bioassays, using the salt-water crustacean Artemia salina Leach, Artemiidae, (conventional 96 microwell plate test and Artoxkit M microbiotest) freshwater Thamnocephalus platyurus Packard, Thamnocephalidae, (Thamnotoxkit F microbiotest), were compared extracts of ten Guatemalan plant species. It was previously observed that five them have anti-Artemia activity. These were: Solanum americanum Mill., Solanaceae, Gliricidia sepium (Jacq.) Kunth ex Walp., Fabaceae, Neurolaena lobata (L.) Cass., Asteraceae, Petiveria alliacea L., Phytolaccaceae, Ocimum campechianum Lamiaceae. The others: Curatella americana Dilleniaceae, Prunus barbata Koehne, Rosaceae, Quercus crispifolia Trel., Fagaceae, Rhizophora mangle Rhizophoraceae, Smilax domingensis Willd., Smilacaceae, do not. All plants without activity had no lethal effects in both assays with A. salina. For not sensitive to G. conventional S. americanum, N. lobata. extracts, except for C. americana, on T. median concentration (LC50) levels this organism all cases substantially lower than those This study revealed is a promising species worth further depth investigation toxicity screening potential medicinal properties.