Cell division and differentiation in protoplasts from cell cultures of Glycine species and leaf tissue of soybean
作者： O. L. Gamborg , B. P. Davis , R. W. Stahlhut
DOI: 10.1007/BF00270107
关键词:
摘要: Protoplasts were isolated from cell cultures of G. soja and tabacina, respectively. The isolation procedure employed Percoll for the separation concentration protoplasts. cultured protoplasts formed cells which developed into embryo-like structures. also leaf tissue soybean cv. Williams 82. Upon culture, regenerated walls divided to form cultures.
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