An in vivo analysis of Miromesh--a novel porcine liver prosthetic created by perfusion decellularization.

摘要: Abstract Background Bioprosthetics derived from human or porcine dermis and intestinal submucosa have dense, homogenous, aporous collagen structures that potentially limit cellular penetration, undermining the theoretical benefit of a “natural” scaffold. We hypothesized Miromesh—a novel prosthetic liver by perfusion decellularization—provides more optimal matrix for tissue ingrowth. Methods Thirty rats underwent survival surgery constituted creation 4 × 1 cm abdominal defect simultaneous bridged repair. Twenty were with Miromesh, 10 non–cross-linked (Strattice). Ten Miromesh all Strattice rinsed in vancomycin solution inoculated 4 colony-forming units green fluorescent protein–labeled Staphylococcus aureus (GFP-SA) after implantation. controls neither soaked nor inoculated. No animals received systemic antibiotics. All euthanized at 90 d an examination their gross appearance before being sectioned quantitative bacterial culture histologic grading. A pathologist scored specimens (0–4) infiltration, acute inflammation, chronic granulation tissue, foreign body reaction, fibrous capsule formation. Results but one rat repaired survived until 90-d euthanization. cultures negative GFP-SA. Of nine explants, none infiltration score >0, consistent poor tissue–mesh interface observed grossly. explants also GFP-SA, seven demonstrated average +2.7 ± 0.8, whereas sterile implants 0.8 ± 1.0. Two inflammation infection on histology. Conclusions generated decellularization provides superior compared dermis.