On the Measurement of Protein Turnover in Animal Cells

摘要: The double isotope technique of Arias, Doyle, and Schimke ((1969) J. Biol. Chem. 244, 3303) with radioactive leucine as precursor has been modified to yield accurate values for rate constants protein degradation. advantages the method are that it is both rapid reproducible in whose half-life being measured isolated only once from one experimental organism obtained degradation comparable those [guanidino-14C]arginine, unlike latter precursor, extensively incorporated into liver protein. turnover population total soluble proteins six homogeneous form rat was studied by means method. majority degraded half-lives between 2 8 days. There a general correlation subunit size subunits multimeric δ-aminolevulinate dehydratase, catalase, ferritin, lactate dehydrogenase at same its quaternary configuration. No intermediate states were found biologically active proteins.